MicroRNAs miR-25, let-7 and miR-124 regulate the neurogenic potential of Müller glia in mice

Citations Over TimeTop 11% of 2019 papers

Abstract

Müller glial cells (MG) generate retinal progenitor (RPC)-like cells after injury in non-mammalian species, although this does not occur in the mammalian retina. Studies have profiled gene expression in these cells to define genes that may be relevant to their differences in neurogenic potential. However, less is known about differences in micro-RNA (miRNA) expression. In this study, we compared miRNAs from RPCs and MG to identify miRNAs more highly expressed in RPCs, and others more highly expressed in MG. To determine whether these miRNAs are relevant to the difference in neurogenic potential between these two cell types, we tested them in dissociated cultures of MG using either mimics or antagomiRs to increase or reduce expression, respectively. Among the miRNAs tested, miR-25 and miR-124 overexpression, or let-7 antagonism, induced Ascl1 expression and conversion of ∼40% of mature MG into a neuronal/RPC phenotype. Our results suggest that the differences in miRNA expression between MG and RPCs contribute to their difference in neurogenic potential, and that manipulations in miRNAs provide a new tool with which to reprogram MG for retinal regeneration.

Related Papers

• → Regulating the formation of Müller glia‐derived progenitor cells in the retina(2024)9 cited
• → Brd activity regulates Müller glia‐dependent retinal regeneration in zebrafish(2023)4 cited
• → Some Problems of Regeneration(1961)14 cited
• → Resources of regeneration in planarians(2006)2 cited
• Newt Lens Regeneration: Role of Oct-4 in Newt Regenerating Tissue and Proteome Analysis of Regeneration Competent Vs. Regeneration Incompetent Cells(2014)