Time-resolved laser studies on the proton pump mechanism of bacteriorhodopsin
Abstract
The research work carried out in 1991 can be classified as follows: (1) Work on the nature of the binding site of Eu{sup 3+} in which a fluorescence technique was used to determine the binding equilibrium constant from the concentration of the free Eu{sup 3+} in equilibrium with the bound ions. (2) The mechanism of the slow deprotonation process of bacteriorhodopsin during its photocycle from the observed temperature and pH dependence of its kinetics. (3) Using the circular dichroism spectrum of bR and its perturbed forms to examine the nature of the primary process as well as the origin of the non-exponential kinetic behavior of its photocycle. (4) Studies of bacteriorhodopsin mutants to identify the important amino acids that are part of the reaction coordinate of the deprotonation process as well as to assign the species that are important in giving rise to UV transient absorption whose origin was controversial.
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