Freeze-Fracture Localization of Filipin-Cholesterol Complexes in the Plasma Membrane of Trypanosoma cruzi
Citations Over TimeTop 13% of 1983 papers
Abstract
The polyene antibiotic filipin was used as a probe for the detection of cholesterol on the P and E faces of the cell body and the flagellar membranes of epimastigotes of Trypanosoma cruzi. When glutaraldehyde-fixed cells were treated with filipin the filipin-cholesterol complexes were homogenously distributed throughout the parasite's plasma membrane. In some cells, mainly on their P faces, such complexes were arranged linearly forming parallel bands with a distance between the bands similar to that existent between the subpellicular microtubules that are attached to the plasma membrane. Filipin-cholesterol complexes were more abundant in the flagellar membrane than in the membrane that enclosed the cell body. No filipin-cholesterol complexes were seen in the membrane of intracellular organelles. Treatment of living epimastigotes with filipin-induced aggregation of cholesterol and intramembranous particles.
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