Observations of Mitosis in Living Cells and Synchrony of Mitosis in Bullera alba

Abstract

SUMMARYPhase-contrast-microscopic observations of living cells undergoing mitosis of Bullera alba (Sporobolomycetaceae) are described, and the observable stages were timed. Nuclear divisions can be partially synchronized in broth culture by starvation followed by resuspension in fresh medium. Of the temperatures tested, growth and synchrony of mitosis are optimum at 21 and 25 C. At these temperatures a maximum of 14% of the population of cells is mitotic, and the population doubles in less than 2 h. Higher temperatures first inhibit mitosis and then inhibit metabolic processes. Lower temperatures slow growth and can arrest the cell cycle in mitosis.

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