Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products
Journal of Visualized Experiments2015Iss. 98, pp. e52412–e52412
Citations Over TimeTop 10% of 2015 papers
Chris Johnston, E. Graeme Robertson, Yvonne Harcus, John R. Grainger, Gillian Coakley, Danielle J. Smyth, Henry J. McSorley, Rick M. Maizels
Abstract
Heligmosomoides polygyrus (formerly known as Nematospiroides dubius, and also referred to by some as H. bakeri) is a gastrointestinal helminth that employs multiple immunomodulatory mechanisms to establish chronic infection in mice and closely resembles prevalent human helminth infections. H. polygyrus has been studied extensively in the field of helminth-derived immune regulation and has been found to potently suppress experimental models of allergy and autoimmunity (both with active infection and isolated secreted products). The protocol described in this paper outlines management of the H. polygyrus life cycle for consistent production of L3 larvae, recovery of adult parasites, and collection of their excretory-secretory products (HES).
Related Papers
- → Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products(2015)103 cited
- → Helminth species richness in wild wood mice,Apodemus sylvaticus, is enhanced by the presence of the intestinal nematodeHeligmosomoides polygyrus(2009)48 cited
- → Cultivation of <em>Heligmosomoides Polygyrus:</em> An Immunomodulatory Nematode Parasite and its Secreted Products(2015)12 cited
- [Changes in the immune response of BALB/c mice coinfected with Heligmosomoides polygyrus and Trichinella spiralis].(2001)
- → Helminths make themselves at home(2021)