Biotinylated boronic acid fluorophore conjugates: Quencher elimination strategy for imaging and saccharide detection
RSC Advances2012Vol. 2(8), pp. 3274–3274
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François D’Hooge, Souad A. Elfeky, Stephen E. Flower, Sofia I. Pascu, A. Toby A. Jenkins, Jean van den Elsen, Tony D. James, John Fossey
Abstract
A biotinylated boronic acid fluorophore conjugate bound to a diol-quencher via a boronic ester linkage demonstrated a fluorescence increase upon exposure to model saccharides. The sensing motif was attached to SuperAvidin microspheres where the sensing regime was imaged by fluorescence microscopy.
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