Modeling of yeast metabolism and process dynamics in batch fermentation
Citations Over TimeTop 10% of 2003 papers
Abstract
Much is known about yeast metabolism and the kinetics of industrial batch fermentation processes. In this study, however, we provide the first tool to evaluate the dynamic interaction that exists between them. A stoichiometric model, using wine fermentation as a case study, was constructed to simulate batch cultures of Saccharomyces cerevisiae. Five differential equations describe the evolution of the main metabolites and biomass in the fermentation tank, while a set of underdetermined linear algebraic equations models the pseudo-steady-state microbial metabolism. Specific links between process variables and the reaction rates of metabolic pathways represent microorganism adaptation to environmental changes in the culture. Adaptation requirements to changes in the environment, optimal growth, and homeostasis were set as the physiological objectives. A linear programming routine was used to define optimal metabolic mass flux distribution at each instant throughout the process. The kinetics of the process arise from the dynamic interaction between the environment and metabolic flux distribution. The model assessed the effect of nitrogen starvation and ethanol toxicity in wine fermentation and it was able to simulate fermentation profiles qualitatively, while experimental fermentation yields were reproduced successfully as well.
Related Papers
- → Sake yeast symbiosis with lactic acid bacteria and alcoholic fermentation(2023)13 cited
- → Adaptation mechanism of yeast to extreme environments: Construction of salt-tolerance mutants of the yeast Saccharomyces cerevisiae(1992)15 cited
- → Physical and biochemical properties of freeze-tolerant mutants of a yeast Saccharomyces cerevisiae(1990)15 cited
- → Cells of the yeast Saccharomyces cerevisiae are transformable by DNA under non‐artificial conditions(2000)3 cited
- [New aspects of research upon the yeast Saccharomyces cerevisiae [PSI+] prion].(2007)